Lucifer yellow was measured using an M5 plate reader (Molecular Probes) at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. 9000-0756. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. LbL films containing TRITC- or FITC-labeled PAA are degraded by DTT and monitored by a fluorometer (SpectraMax M5 Plate Reader, Molecular Devices). Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. 5 hours after an initial 10-minute incubation period. and 72 h using the CCK-8 protocol with absorbance readings at 450 nm measured with a SpectraMax M5 plate-reader (Molecular Devices. $4,995. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. 304 signal level saturation. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 6. To determine whether each drug had proved. 0 adjusted at rt containing 10% v/v DMSO) (100 μL) and a 2- or 5-fold dilution was. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. Molecular devices spectramax™ m5 microplate reader w/ warranty (svcSpectramax® m5/m5e multimode plate reader, molecular devices Molecular devices announces next-generation spectramax minimax 300Microplate spectramax m5 molecular reader devices contract warranty svc available labx biotech laboratory readers science. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The SpectraMax® ABS Plus Microplate Reader can run . The plates were centrifuged at the end of incubation, and fluorescent signals in supernatants were measured using a SpectraMax™ M5 microplate reader, with excitation at 485 nm and emission at 520 nm. 25 pM (0. On microplate readers,. 9. Luminescence was measured with an integration time of 1000 ms per well on a SpectraMax M5 plate. 09 in One Micro Manipulator Reader software. On the day of the assay, the plate was equilibrated to room temperature, and then 100 μL reconstituted Steady-Luc working solution containing D-Luciferin was added to each well. 3. T o enable accurate quantita-tion, initially standard curves were plotted f or both Pi and. Activity assays were performed in clear, flat-bottomed 96-well plates and the decrease in absorbance at 340 nm was recorded using a SpectraMax M5 plate reader (Molecular Devices). Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Incubation. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 1. Microplate Reader Shelf. 5. Bacterial growth was carried out at 25 °C in a 200 l volume withconstantshakingat450rpm. The EC50 values were calculated from the absorbance values vs. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 5. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. 6 for further experiment, so I'm confused which i would choose for OD value determination. The average 526/470 ratio for the sample aliquots. To the OP, if you are familiar with VAG-COM, you know that RS232-based VAG-COM cables are in the $14 range on eBay. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Specifications General -Detection mode Fluorescence, Luminescence -Read method End point, kinetic. Fluorescence Microplate Reader Comparison Chart. g. com 9. Cells were seeded at a density of 1,000 cells/well (A549: 500 cells/well). Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. Wide range of applications. Reader Documentation Version No. . Turn the reader on and secure the USB cable. The Infinite and Spark multimode microplate readers are modular and upgradeable. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. Typically the system can detect absorbance, luminescence, fluorescence,. Wells with an OD 600[0. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. The StakMax stacker also allows users to feed plates into the loading area manually. 72 MB. Clear Filters. Imaging opens up a range of applications for cell-based assays that cannot be performed on a standard plate reader. Fast protein liquid chromatography (FPLC) was performed as the last protein purification step on a Bio-Rad BioLogic DuoFlow using a HighPrep TM 26/60 Sephacryl TM S-200 High Resolution column. Plate chamber design of the SpectraMax category 1 microplate readers. Results: In the presence of Cliradex ®, Johnson’s® Baby Shampoo, OCuSOFT Lid Scrub®Before termination, 2 μM of Rhodamine-123 was added in each well and plates were again incubated at 37 °C for 30 min. 1 M glycine-phosphate-citrate buffers with 0. 17. Wide range of applications The VersaMax reader covers a wide range of applications:To monitor the increase rate, fluorescence measurement is then performed at 37°C at 10-s intervals for 10 min with Ex/Em 450/510 nm in the plate reader (Spectramax M5 plate reader, Molecular Devices, For each bacterial sample, we performed the reactions in duplicate and averaged the results. Simply unpack it, plug it in, and begin your experiments. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Learn how to use the SpectraMax M5 SpectraMax M4 SpectraMax M5 SpectraMax M5e Multi-Mode Microplate Reader, a device that can read microplates and cuvettes with different modes and settings. 5 hours after an initial 10-minute incubation period. Molecular Devices M5 plate readers; Multiple liquid bulk dispensers (Wellmate, Multidrop Combi) Price List. Products. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. The fluorescence was then measured with a fluorescence plate reader (SpectraMax M5; Molecular Devices, Inc. This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. 0200-7027. 1. 5 mL, pH 7. 9. 25% (v/v) AlamarBlue reagent was then added to cells and incubated in normal cell culture conditions for 1 h, protected from light. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications in the clinical laboratory. 2. For a higher . Figure 9. Description Figure 1. Fluorescence values were used to calculate the percent inhibition of enzyme activity relative to maximal activity, PTP4A3 in the absence of inhibitor, and maximal inhibition, PTP4A3 in the presence of 2 mM Na 3 VO 4. Catalog number: 165306. 3. Page 1 of 8 . Find regional support. RUO. Thus, the. CD spectra were recorded using a Jasco-715 circular dichroism spectrophotometer at 20 °C. We use the SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). Cell–cell fusion assays were also performed by transient transfection of pHXB2-env plasmid [34] along with pRev and pTat plasmids into 293T cells and these were added to the receptor. 3465 or email us. pH-Dependent Solubility. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Bioluminescence assays showed that the engineered Luc-Cys was fully active when compared with 6 × His-NanoLuc prepared in our previous work (Zhang et al. 19 , 25 Human umbilical cord endothelial cells (ATCC, Manassas, VA) were grown in 24-well plates coated with. The IC50 was calculated using the DRC package in the R software. Cell apoptosis assay. Defining the role of the polyasparagine repeat domain of the S. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. Detection modes are available in UV-Vis absorbance, fluorescence, and. coli cells were purchased from New England Biolabs (Ipswitch, MA). The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. The plate can be shaken (icon right of thermometer, and see automix option in setup). Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The emission of roGFP2 was measured between 500 and 530 nm after excitation at 405 nm and 488 nm every 30 s in a SpectraMax M5 plate reader (Molecular Devices). M-5 The M-5 multitronic unit, or the M-5 computer, was an advanced multitronic computer system and prototype created by Doctor Richard Daystrom during. Riss, Germany) at 330 nm and room temperature for 30 min. 2. 7°C and the bottom to 36. 600 in a Molecular Devices SpectraMax M5 plate reader at 30˚C with shaking prior to each reading. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. Instructions on the proper use of the SpectraMax M5 Plate Reader. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. ZERO BIAS - scores, article reviews, protocol conditions and more. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Fe standards require only about 30 minutes but can be kept in 1x working solution for at least a week withoutAfter 15 min, the absorbance at 550 nm was recorded using a Molecular Devices SpectraMax M5 plate reader. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. 5: Schematic of the back panel of a reader. For. wavelength and 595 nm emission wavelength by Spectramax M5 plate reader (Molecular Devices). DH5α and BL21 (DE3) chemically competent E. Information on how to reserve the plate readerBioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. Please contact Tatiana to reserve the plate reader time: tnl1@psu. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. The plate was washed and an anti-rabbit peroxidase-conjugated secondary antibody (Jackson Immunoresearch) was applied at a 1:3000 dilution in PBS. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Cultures were grown at 30°C and 900 rpm for 24 h, then OD600 was measured on a SpectraMax M5 plate reader to determine bacteriostatic potential. Luminescence, Fluorescence Intensity, UV-Visible Absorbance, Filtered Luminescence, BRET and FRET. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. 72 MB. Following color development, absorbance was measured at 450nm using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA), and BDNF concentrations in samples were calculated using SoftMax Pro software. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. 7°C (gradient heating design). SpectraTest ABS2 Absorbance Validation Package performance tests. 2% acetamide. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. The peptide concentration was. The fluorescence was measured using a SpectraMax M5 plate reader at 358 nm excitation and 455 nm emission. Extracting viral DNA from 4 x 109 copies of the AAV8 preparation ( nominally 5 ng ssDNA)The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. PMC full text: Oncotarget. ThT fluorescence was measured at 444 and. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. 1. 17. The TRITC and FITC labeling process is conducted as in a previous study . 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Absorbance was measured at 563 nm using a SpectraMax M5 plate reader (Molecular Devices). The Back Panel Figure 1. Copia stocks a full line of used microplate readers. The following document is. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. You can also interpret your results using integrated data analysis software. S. Options include top and bottom detection, temperature control and reagent injector. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. BioTek 50TS Plate Washer; Chemidoc MP; C1000 Thermal Cycler; Fragment Analyzer 5200; Genesys 10S UV-Vis Spectrophotometer; Guava EasyCyte 5HT; KingFisher Flex; MinION; MiSeq; Nanodrop; QuantStudio 3D; QuantStudio 5; Qubit 4; Savant DNA 120 SpeedVac SpectraMax M5 Plate Reader; StepOnePlus qRT PCR; ST1R Centrifuge;. The plate was incubated for 4 h and the absorbance of the solubilized formazan was measured on a Molecular Devices Spectramax M5 plate reader at 570 nm. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. Luminescence was measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA) after the addition of nano-Glo substrate (Promega, Madison, WI) in accordance with the manufacturer’s directions. Microtiter plates (Corning 3690; 96-well half area plates) were coated overnight at. 5. 25 pM (0. 12. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). The SpectraMax 190 reader can be integrated with Molecular . 5 × 10 5 counts/fmol, thus the suitable enzyme range for bioluminescence measurement is 0. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Fuchs1,2,3* 1 Department of Biology, Tufts University, Medford, MA 02155, USA 2 Institute for Protein Innovation, Boston, MA 02115, USA 3 Program in Cellular and Molecular Medicine,. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Then the plate was incubated at 37 °C for 2 h. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Spend a little more and you can get an FTDI-based USB cable. 01 M HCl was added, and absorbance was read at 570 nm using a Spectramax M5 plate reader (Molecular Devices). Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. #3635 or Greiner cat. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. The password is. Apoptosis was measured by annexin V–fluorescein isothiocyanate (FITC)/7-amino-actinomycin D (7-AAD) labeling followed by fluorescence flow cytometry as previously described. Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA quantification or protein quantification. RoHS Compliant. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected] H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. Accordingly there are different configurations possible. Bradford assays were conducted in 96-well plate format and absorption values recorded on a Molecular Devices SpectraMax M5 plate reader (Sunnyvale, CA). After the plate is read, data will appear in the plate section: 11. The % PEG midpt and apparent solubility values (thermodynamic activity) were calculated as described previously. 2 mg/mL using 2 different UV-visible spectrophotometers, a SpectraMax M5 plate reader, and a NanoDrop instrument. Learn how to use the SpectraMax M5 SpectraMax M4 SpectraMax M5 SpectraMax M5e Multi-Mode Microplate Reader, a device that can read microplates and cuvettes with. This unit must survive. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. RNA for the RNA-Seq experiment was obtained exactly as described previously for the quantitative RT-PCR. The absorbance at a wavelength of 540 nm was quantified using a Spectramax M5 plate-reader (Molecular Devices) to determine the amount of gamma glutamyl hydroxamate, indicative of active enzyme. Highly selective for dsDNA over ssDNA or RNA. ScanLater Western Blot Detection Cartridge. 3 Kg) Regulatory Regulatory CE and TUV marked. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Cells were collected and fixed with precooled 95 % ethanol at 4 ºC for 12 h. Relative fluorescence was quantified by dividing the fluorescence by the OD 600 reading of the culture. Kojic acid was used as a positive control. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. HTRF-compatible SpectraMax Multi-Mode Readers. Viability assays were performed in 384-well microtiter plates with biologic and technical duplicates. We use Spectramax M5 microplate reader in our lab. The percent relative binding potency was calculated by dividing the reference material. Fluorescein 0. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 2 software for data reduction (Molecular Devices, Sunnyvale, CA). The antibiotic bacitracin A has been used as an inhibitor of thiol isomerases, including ERp5, in the insulin reductase assay . Most papers they cultured bacteria to OD600>0. 6. Cultures were grown for 8 h, and then fluorescence was quantified (excitation, 475 nm; emission, 510 nm) using an M5 SpectraMax plate reader (Molecular Devices). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. g. 0 μM wild-type R3/I5. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. Even better, they require MINIMAL modification to. Cytation 5 extends the legacy of Agilent BioTek multimode plate readers with a modular and upgradable imaging mode. Flow cytometry. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 9. For a Price List, please call (858) 646-3100 ext. doi: 10. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues are proactively addressed. The concentration of MMP-9 was measured using R&D System’s ELISA Kit (Minneapolis, MN) on a Spectramax M5 plate reader equipped with SoftmaxPro v5. 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. 9000-0756. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. When a feature or capability applies to only certain readers, this. Multimode microplate reader equipped with latest detections like fluorescence, absorbance etc. The drawing shows a standard microplate of 15 mm height covered with a lid. The inhibitory rate of ingredients was calculated as follows: Inhibition (%) = (FI of control – FI of treatment) / FI of control × 100. xlsx file, with or without the averages and normalizations. Cells stably expressing wild-type or E633K p110β were. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. 10. After this time period, the plate was read for fluorescence intensity at ex/em wavelengths of 532/590 nm, on a SpectraMax M5 plate reader. Published Dec 16, 2020 | Updated Dec 16, 2020. Major items of equipment include the following: Agilent MR400 400 MHz two-channel PFG NMR. siRNA oligonucleotides targeted specifically to rat P2X 7 were used in this experiment. Fluorescence, brightfield, color brightfield, and phase contrast imaging are available with the automated cell imager. The optical density (OD) at 450 nm was recorded by SpectraMax M5 plate reader (Molecular Devices, CA). 5: Schematic of the back panel of a reader. Fluorescein 0. 1. Reactions were performed on a 384-well plate, with release of 6-FAM dye monitored by fluorescence at 517 nm using a SpectraMax M5 plate reader (Molecular Devices) with excitation at 495 nm for 60. After MMP9-antibody complex formation, substrate (20 μM for human and mouse assays, 10 uM for rat) was added and fluorescence was monitored in kinetic mode at 37°C on either a SpectraMax M2 or M5. 0 mM of NADH per min at pH 8. 10569C 2 C 12 cells were treated exactly as described for free [Mg 2+] determination, except that MgG was replaced by 5 μM safranine O. M5 plate reader at 595 nm. 5. After the plate is read, data will appear in the plate section: 11. reader. Tablet PC. How-to-use guide for the M5 plate reader and SpectraMax Software in the CMB. A solution for low volume applications. Turn on the SpectraMax M5 Multi-Mode Microplate Reader. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. LbL films are immersed into 20 mM DTT in PBS buffer (2. An emission wavelength of 535 nm was used for carboxy-H2DCFDA and 610 nm was used for DHE and MitoSOX ™. 1% Tween-20 and bound secondary was detected using Ultra TMB (Thermo Fisher); signals were read on a Molecular Devices Spectra Max M5. High tolerance for contaminants. Plates were incubated at 37°C for 6 hours and then equilibrated to room temperature for 30 min. Shop now. begin reading microplates with seven mouse clicks. The multi-mode. Published Dec 16, 2020 | Updated Dec 16, 2020. The fluorescence intensity was measured using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, Calif) with excitation/emission at 485/530 nm. Detection modalities. When the user applies a set temperature of 37°C, the top is heated to 37. Endothelial permeability was also measured in vitro, using a transwell assay. A sensitivity level of 0. 4. doi: 10. There is currently no charge for using this plate reader. 5. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Temperature can be set to 4º above ambient to 45°C (thermometer icon) on main toolbar. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 흡광, 형광, 발광 중 하나의 측정 모드만 이용할 수. Reader Documentation Version No. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. The sample is typically placed in a cuvette through which light is sent horizontally. In the M2, M5 and Plus 384, the light. [1] AnkerMake M5 is capable of printing at a speed of 500 mm/s and acceleration of 5,000 mm/s² with the new Fast Mode upgrade. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Viability was evaluated using the Cell Titer Glo Assay (Promega) and luminescence was read on a SpectraMax M5 plate reader (Molecular Devices). Data reduction and calculation were performed with MSD Discovery workbench software (v 3. Duplicate wells were tested for each condition, and data were graphed as a percent of control in GraphPad Prism (GraphPad Software). Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. Human T-cells were cultured at 1 × 10 6 /mL for 5 days with plate-bound 10 μg/mL anti-CD3 and soluble 1 μg/mL anti-CD28. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. A SpectraMax M5 plate reader was able to continuously incubate the microtiter plate at 37°C and measure the fluorescence (λex/λex= 529/600 nm) every 5 minutes for 1. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. HSL_EQ_004: Use and Maintenance of a BioTek Plate Washer in the HPV Serology Laboratory 3. Turn on Plate Reader and wait for it to complete its start-up routine. 40266 Email: [email protected]) at room temperature. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. Standards and QCs are composed of a. In my opinion one the best plate readers which I used till now is Molecular Devices SpectraMax M5 Plate reader. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Protocol for Qubit DNA BR Assay. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Nonspecific binding was determined by competition with 1. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. com 10. 2000) supplemented with each chemical com-pound with the final concentration of 10 mmol l-1. The colorimetric reaction was stopped with 2 M sulfuric acid, and absorbance was measured at 450 nm using a SpectraMax M5 plate reader (Molecular Devices). Developed with Promega reagents to provide a. SpectraMax microplate reader with UV absorbance detection (Molecular Devices) UV–transparent microplates Disposable: Corning cat. Read More. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Fast protein liquid chromatography (FPLC) was performed as the last protein purification step on a Bio-Rad BioLogic DuoFlow using a HighPrep TM 26/60 Sephacryl TM S-200 High Resolution column. The plate reader. Appropriately. SpectraMax Injector Cartridge with SmartInject™ Technology. The solution was shaken at 37°C and 1200 rpm for 3 days. Published Dec 16, 2020 | Updated Dec 16, 2020. Dimensions 20" D x 16. 51 L/g). Developed with Promega reagents to provide a. The assay was performed on HDL collected. To detect the luminescent signals, 100 μl of each dilution was added into a 96-well plate before adding 50 μl of LumiGLO Peroxidase Chemiluminescent substrate (KPL, 54-71-00) to measure luminescent intensity by a SPECTRAmax M5 plate reader. How-to-use guide for the Nanodrop Spectrophotometer. Lucifer yellow concentrations in the donor and acceptor wells were measured in the last of this incubation. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Linear, Orbital Slow, and Orbital Fast. Caspase activity measurementAfter addition of the 50-fold diluted nanoluciferase substrate (Promega, diluted in phosphate buffered saline, 50 μl/well), the bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular. In general, reading fluorescence from the top is more sensitive than reading from the bottom. Lastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. com 9. Microplate Readers; SoftMax Pro Software; Microplate Stacker and Washer; Cellular Imaging Systems; Clone Screening; FLIPR; AXON Patch-Clamp; Assay Kits. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. Uncontrolled Copy for Training and Reference Purposes Only . For the Evans blue (EB) dye extravasation test, 3,8. siRNA (750 pmol) was transfected into NRK-49F (1 × 10 6 cells) using a Nucleofector Kit V and the Amaxa Nucleofector. Table of Contents. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 0 out of 5 $ 3,799. Absorbance was measured at 405nm on a Molecular Devices SpectraMax M5 plate reader. For experiments with TGX-221, the cells were treated with 200 nM of TGX-221 throughout the duration of the experiment. The Back Panel Figure 1. I have a 2002 M5 and my medium-deluxe Harbor Freight reader doesn't talk to the BMW whatsoever. 8 cm x 41. SpectraTest® Validation Plates User Manual & Protocol Download Page. After mixing with freshly diluted substrate (50 μl well −1), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) under luminescence mode.